Product name: β-Nicotinamide Mononucleotide

CAS: 1094-61-7

Molecular formula: C11H15N2O8P

EINECS: 214-136-5

β-Nicotinamide mononucleotide Properties

Introduction

Nicotinamide mononucleotide, also known as β-nicotinamide mononucleotide (NMN), is a synthetic substrate of coenzyme I. At the same time, NMN is also used in anti-aging research. Studies have shown that β-NMN can also regulate insulin secretion and affect the expression level of mRNA. β-NMN has broad application prospects in the field of medical treatment. β-Ninotinamide mononucleotide is a product of the extracellular nicotinamide phosphoribosyltransferase (eNAMPT) reaction and a key NAD+ intermediate. It improves glucose tolerance by restoring NAD+ levels in HFD-induced T2D mice. It also enhances hepatic insulin sensitivity and restores gene expression related to oxidative stress, inflammatory response and circadian rhythm partly through SIRT1 activation.

Properties

β-Nicotinamide mononucleotide is white to slightly yellow in appearance, a crystalline powder with no noticeable odor.

Source

Nicotinamide mononucleotide (NMN) is a substance inherent in the human body and is also rich in some fruits and vegetables, including broccoli and cabbage.

Uses

In mammals, β-nicotinamide mononucleotide is generated from nicotinamide (Nam) under the catalysis of Nampt (a protease in the body), and then nicotinamide mononucleotide generates NAD+ under the catalysis of nicotinamide mononucleotide adenylyltransferase. Nicotinamide mononucleotide is a direct way to supplement NAD+. Nicotinamide mononucleotide achieves the effect of reversing aging by significantly improving the ability to repair DNA damage in cells. Nicotinamide mononucleotide will be converted into "nicotinamide adenine dinucleotide (NAD)", which is essential for energy metabolism in the body. In mouse experiments, it was confirmed that nicotinamide mononucleotide can activate a gene called acetylase in the body, thereby exerting effects such as prolonging life and treating diabetes. NAD is a substance that the human body can originally produce. Studies have confirmed that the content of NAD in the body decreases with age.

Applications

β-Nicotinamide mononucleotide is the precursor of NAD+, a cofactor of longevity proteins in the human body. NAD+ is an important coenzyme in the tricarboxylic acid cycle, promoting the metabolism of sugars, fats, and amino acids, and participating in the synthesis of energy; NAD+ is also the only substrate of coenzyme I consuming enzymes (the only substrate of DNA repair enzyme PARP, the only substrate of longevity protein Sirtuins, and the only substrate of cyclic ADP ribose synthetase CD38/157). NAD+ is involved in all aspects of human metabolism and is a key coenzyme. Without NAD+, metabolism will not work. Elderly people lack NAD+, so various major and minor problems come. By supplementing NAD+, comprehensive anti-aging can be achieved. Preclinical studies have shown that NMN has multiple pharmacological activities in cardiac and cerebral ischemia, Alzheimer's disease, type 2 diabetes and obesity caused by diet and age. As a precursor drug of NAD+, NMN mainly synthesizes NAD+ in the body to exert pharmacological effects, all of which are related to the lack of NAD+.

Preparation

Add 150ml of phosphate buffer solution to a 500ml four-necked bottle. Add 2g magnesium chloride, 10g ATP, 7g ribose, and 10g nicotinamide at one time, and stir at 35℃. Detect the pH value of the reaction system, and adjust the pH to 8.0 with 10% Na2CO3 solution, then add 1g RK enzyme powder and 2g NAMPT enzyme powder, and stir to dissolve. Stir the reaction at 37℃ and 200rpm, and monitor the conversion rate of the reaction by liquid chromatography-mass spectrometry. After 6 hours of reaction, it is detected that ATP has been consumed and the reaction is stopped. Through further filtration, HZ-818 macroporous resin adsorption, freeze drying, ethanol and water recrystallization, β-nicotinamide mononucleotide-nicotinamide mononucleotide NMN can be obtained with a yield of 70%

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